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phospho tie2 ser1119  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho tie2 ser1119
    Phospho Tie2 Ser1119, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+tie2+ser1119/Phospho-Tie2+(Ser1119)+Antibody/pm39892310-68-14-26
    Average 94 stars, based on 11 article reviews
    phospho tie2 ser1119 - by Bioz Stars, 2026-08
    94/100 stars

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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and <t>TIE2</t> transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).
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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and <t>TIE2</t> transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).
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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and <t>TIE2</t> transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).
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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and <t>TIE2</t> transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).
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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and <t>TIE2</t> transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).
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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and <t>TIE2</t> transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).
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    FIGURE 5. METTL3 regulates m6A modification in MMP2 and TIE2 transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).

    Journal: Investigative ophthalmology & visual science

    Article Title: METTL3-Mediated RNA m6A Modification Regulates the Angiogenic Behaviors of Retinal Endothelial Cells by Methylating MMP2 and TIE2.

    doi: 10.1167/iovs.64.13.18

    Figure Lengend Snippet: FIGURE 5. METTL3 regulates m6A modification in MMP2 and TIE2 transcripts. (A) Schematic representation outlining the strategy for identifying potential targets influenced by METTL3. (B) MeRIP-qPCR assay delineating the relative m6A enrichment in MMP2, CDC42, TIE2, and PTEN transcripts in METTL3 siRNA-treated HRMECs relative to the negative control group (n = 3/group). (C) RT-qPCR quantification of MMP2 and TIE2 mRNA expression in HRMECs post-transfection with METTL3 or NC siRNA (n = 3/group). (D) Protein expression levels of MMP2 and TIE2 in HRMECs post METTL3 siRNA or NC transfection, assessed under both normoxic (21% O2) and hypoxic (1% O2) conditions using Western blot. (E) Densitometric analysis of the protein bands from the Western blot (n = 3/group).

    Article Snippet: These membranes were incubated overnight at 4°C with specific primary antibodies: METTL3 (1:1000; Abcam), METTL14 (1:1000; Invitrogen), WTAP (1:1000; CST), FTO (1:1000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), ALKBH5 (1:1000; Novus Biologicals, Littleton, CO, USA), MMP2 (1:1000; CST), TIE2 (1:1000; CST), HIF-1α (1:1000; Abcam), and β-ACTIN (1:1000; Santa Cruz Biotechnology).

    Techniques: Modification, Negative Control, Quantitative RT-PCR, Expressing, Transfection, Western Blot

    FIGURE 6. Consequences of MMP2 or TIE2 inhibition on HRMEC angiogenic activities. (A) In vitro Matrigel-based angiogenesis assay illustrating the tube formation capabilities of MMP2, TIE2, and NC siRNA-transfected HRMECs under normoxic conditions. (B) Quantita- tive data reflecting the number of nodes and cumulative tube length from the in vitro Matrigel-based angiogenesis assay (n = 3/group). (C) Representative imagery from the transwell assay assessing endothelial migration potential. (D) Quantitative assessment of migratory cells derived from transwell assays (n = 3/group). (E) Under hypoxic conditions, the in vitro Matrigel-based angiogenesis assay highlights the tube formation proficiency of MMP2, TIE2, or NC siRNA-transfected HRMECs. (F) Quantitative evaluation of the in vitro Matrigel assays under hypoxia (n = 3/group). (G) In the context of hypoxia, the transwell assay unveils the migratory capabilities of HRMECs post-transfection with MMP2, TIE2, or NC siRNA. (H) Quantitative data detailing the number of migrating cells from the hypoxic transwell assays (n = 3/group). Scale bar: 200 μm.

    Journal: Investigative ophthalmology & visual science

    Article Title: METTL3-Mediated RNA m6A Modification Regulates the Angiogenic Behaviors of Retinal Endothelial Cells by Methylating MMP2 and TIE2.

    doi: 10.1167/iovs.64.13.18

    Figure Lengend Snippet: FIGURE 6. Consequences of MMP2 or TIE2 inhibition on HRMEC angiogenic activities. (A) In vitro Matrigel-based angiogenesis assay illustrating the tube formation capabilities of MMP2, TIE2, and NC siRNA-transfected HRMECs under normoxic conditions. (B) Quantita- tive data reflecting the number of nodes and cumulative tube length from the in vitro Matrigel-based angiogenesis assay (n = 3/group). (C) Representative imagery from the transwell assay assessing endothelial migration potential. (D) Quantitative assessment of migratory cells derived from transwell assays (n = 3/group). (E) Under hypoxic conditions, the in vitro Matrigel-based angiogenesis assay highlights the tube formation proficiency of MMP2, TIE2, or NC siRNA-transfected HRMECs. (F) Quantitative evaluation of the in vitro Matrigel assays under hypoxia (n = 3/group). (G) In the context of hypoxia, the transwell assay unveils the migratory capabilities of HRMECs post-transfection with MMP2, TIE2, or NC siRNA. (H) Quantitative data detailing the number of migrating cells from the hypoxic transwell assays (n = 3/group). Scale bar: 200 μm.

    Article Snippet: These membranes were incubated overnight at 4°C with specific primary antibodies: METTL3 (1:1000; Abcam), METTL14 (1:1000; Invitrogen), WTAP (1:1000; CST), FTO (1:1000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), ALKBH5 (1:1000; Novus Biologicals, Littleton, CO, USA), MMP2 (1:1000; CST), TIE2 (1:1000; CST), HIF-1α (1:1000; Abcam), and β-ACTIN (1:1000; Santa Cruz Biotechnology).

    Techniques: Inhibition, In Vitro, Angiogenesis Assay, Transfection, Transwell Assay, Migration, Derivative Assay

    FIGURE 7. Schematic diagram illustrating the METTL3-mediated m6A modification of MMP2 and TIE2 transcripts and its consequential regulation of angiogenic activities in retinal endothelial cells. SAM, S-adenosylmethionine, methyl donor; SAH, S-adenosylhomocysteine.

    Journal: Investigative ophthalmology & visual science

    Article Title: METTL3-Mediated RNA m6A Modification Regulates the Angiogenic Behaviors of Retinal Endothelial Cells by Methylating MMP2 and TIE2.

    doi: 10.1167/iovs.64.13.18

    Figure Lengend Snippet: FIGURE 7. Schematic diagram illustrating the METTL3-mediated m6A modification of MMP2 and TIE2 transcripts and its consequential regulation of angiogenic activities in retinal endothelial cells. SAM, S-adenosylmethionine, methyl donor; SAH, S-adenosylhomocysteine.

    Article Snippet: These membranes were incubated overnight at 4°C with specific primary antibodies: METTL3 (1:1000; Abcam), METTL14 (1:1000; Invitrogen), WTAP (1:1000; CST), FTO (1:1000; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), ALKBH5 (1:1000; Novus Biologicals, Littleton, CO, USA), MMP2 (1:1000; CST), TIE2 (1:1000; CST), HIF-1α (1:1000; Abcam), and β-ACTIN (1:1000; Santa Cruz Biotechnology).

    Techniques: Modification